Increased abundance of frost mRNA during recovery from cold stress is not essential for cold tolerance in adult Drosophila melanogaster.

摘要: Frost (Fst) is a candidate gene associated with the response to cold in Drosophila melanogaster because Fst mRNA accumulation increases during recovery from low temperature exposure. We investigated contribution of expression chill-coma time, acute tolerance and rapid hardening (RCH) adult D. melanogaster by knocking down Fst mRNA using GAL4/UAS-mediated RNA interference. In this experiment, four UAS-Fst one tubulin-GAL4 lines were used. predicted that if essential for phenotypes, flies levels should be less tolerant than normal cold-induced Fst mRNA. Cold-induced abundance time not negatively correlated male or female flies. Survival 2 h exposures sub-zero temperatures knockdown was lower control line. Moreover, pretreatment increased survival severe exposure regardless level stress, suggesting RCH. Thus, measured as stress RCH D. melanogaster.