Sparse force-bearing bridges between neighboring synaptic vesicles.

摘要: Most vesicles in the interior of synaptic terminals are clustered clouds close to active zone regions plasma membrane where exocytosis occurs. Electron-dense structures, termed bridges, have been reported between a small minority pairs neighboring within clouds. Synapsin proteins implicated previously, but existence bridges as stable structures vivo has questioned. Here we use electron tomography show that present less frequent synapsin knockouts compared wildtype. An analysis distances neighbors wildtype tomograms indicated strong enough resist centrifugal forces likely induced by fixation with aldehydes. The results confirm and involved formation or stabilization.