Morphology of long-term denervated rat soleus muscle and the effect of chronic electrical stimulation.

摘要: 1. Rat soleus muscles were denervated for 6-10 months; some of these chronically stimulated the last 3-8 weeks before recording. The fixed at physiological lengths and embedded in epoxy resin. 2. Sections light microscopy stained with p-phenylenediamine. Denervation reduced mean cross-sectional area fibres to 3% controls (peak 20 microns2). areas had a peak 70 microns In micrographs muscles, total number appeared be reduced; however not all could identified (see paragraph (3)). 3. electron microscope, many showed disarrayed myofilaments. Few more or less normal cross-striations. Muscle as small 1 micron diameter seen. smallest did contain Some unequivocally necrotic 4. Most an almost sarcomere pattern. Scattered throughout muscle single 2 diameter, but well organized recognized microscope. necrotic. Often serially arranged. Satellite cells prominent. 5. It is concluded that long-term rat original are lost those seen result repeated cycles regeneration necrosis. Stimulation maintains sarcomeres probably prevents secondary degeneration