Experimental vaccination against Mycoplasma agalactiae using different inactivated vaccines.

摘要: Abstract Five sets of vaccines were prepared using Mycoplasma agalactiae washed cultures inactivated with phenol (1), formalin (2), heat-treatment (3), sodium hypochlorite (4) and saponin (5). All sets, except for saponin-vaccine, adjuvated aluminium hydroxide. groups six sarda ewes inoculated twice before pregnancy, once during pregnancy challenged the lactation period. Monthly blood samples taken from vaccinated sheep 12 controls: sera assayed by immunoblotting, ELISA growth inhibition tests. Four control infected intracanalicular route pooled mycoplasmas at concentrations 10 4 , 5 6 7 CCU. The challenge involved milk to contaminate remaining sheep. controls some 2, 3 developed contagious agalactia. Ewes phenol- saponin-inactivated resisted experimental challenge. These results suggest that these two are effective their use could limit diffusion M. infection.