Efficiency of inactive vaccines against contagious agalactia in Brazil

摘要: This paper aims to evaluate the efficiency of three inactive vaccines against contagious agalactia prepared with samples Mycoplasma agalactiae isolated in Brazil and different adjuvants. Vaccine 1 adsorbed aluminum hydroxide was administered 23 goats (Gc1) 13 sheep (Gov1); vaccine 2 containing Montanide IMS-2215-VG 22 (Gc2) 12 (Gov2) 3, Gel-01 (Gc3) (Gov3). All animals were negative for Ma at indirect ELISA received two doses 2mL each, subcutaneously, within a 21 day interval. Five from each species used as control. Seventy-five days after booster, four vaccinated group control challenged 5mL culture 107cfu/mL, orally through immersion female's udder lactation. The serological response analyzed during vaccination (0 21) 51, 81, 111, 141 171 vaccination. collection analysis conducted challenge (D0) 7, 14, 21, 28, 35, 42, 49 56 challenge. induced production antibodies, having no significant statistical difference (p<0.05). Animals groups Gc1, Gc2 Gov2 developed higher levels compared other After challenge, presented an increase regional lymph nodes conjunctivitis, mastitis arthritis. In animals, discrete conjunctivitis congestion episcleral veins observed. It is concluded that protective antibodies sheep, sufficient clinical protection submitted experimental infection, indicating its use on prevention agalactia.