Bacterial invasion of the uterus and oviducts in bovine pyometra

摘要: Pyometra is a common disease of cattle that causes infertility and thereby financial losses to the industry. Bacteria involved in development progression pyometra have been investigated by microbial culture but their tissue invading abilities, which an important aspect bacterial pathogenicity lesions, not investigated. Bacterial invasion uterus oviducts was studied 21 cows diagnosed with at time slaughter applying fluorescence situ hybridization using probes targeting 16S ribosomal RNA Fusobacterium necrophorum, Porphyromonas levii, Trueperella pyogenes overall domain Bacteria. necrophorum P. levii were found invade endometrium, especially if endometrium ulcerated, penetrated deep into lamina propria. These species co-localized within thus indicating synergism. did uterine tissue. In addition endometrial most also had salpingitis without significant oviductal wall.