Involvement of tyrosine kinases in the activation of human peripheral blood neutrophils by granulocyte-macrophage colony-stimulating factor.
作者： SR McColl , JF DiPersio , AC Caon , P Ho , PH Naccache
DOI: 10.1182/BLOOD.V78.7.1842.1842
关键词:
摘要: The aim of the present study is to evaluate involvement human neutrophil tyrosine kinase(s) in signal transduction mechanism granulocyte-macrophage colony-stimulating factor (GM-CSF). Stimulation neutrophils with GM-CSF resulted a time- and dose-dependent phosphorylation several proteins having estimated molecular weights approximately 40, 55, 74, 97, 118, 155 Kd, detected by immunoblot using monoclonal antibody directed against phosphotyrosine. GM-CSF-induced was inhibited dose- time-dependent manner kinase inhibitor erbstatin. Using this inhibitor, we were able correlate functional effects on neutrophils. Pretreatment erbstatin before incubation completely intracellular alkalinization, downregulation leukotriene B4 receptor, enhancement fMet-Leu-Phe-induced calcium mobilization, as well accumulation mRNA for proto- oncogene c-fos. Taken together, these data suggest that activation plays critical regulatory role both stimulation priming function GM-CSF.
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