MALDI mass spectrometry imaging of proteins exceeding 30 000 daltons

作者: Alexandra Van Remoortere , Maxence Wisztorski , René Van Zeijl , André Deelder , Liam McDonnell

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摘要: Background Since its introduction 10 years ago by Caprioli and associates, MALDI mass spectrometry imaging has enabled spatial analysis of drugs, lipids, peptides, polypeptides. In polypeptides, the detectable range is limited to small proteins with a less than 25 kDa. This limitation, as many proteins, including cytokines, growth factors, enzymes, receptors have molecular weights, exceeding present work, we report development novel strategy observe higher up 30 Material/methods We investigated sample preparation methods based on hexafluoroisopropanol (1,1,1,3,3,3-hexaluoro-2-propanol) 2,2,2-trifluoroethanol solvents for protein solubilization optimized high-mass proteins. Results were, first time in imaging, able detect 70 kDa directly from tissue. These developments indicate future avenues which sensitivity can be further improved. applied these ovarian cancer demonstrate that are similar obtained using 2D gel analyses. Conclusions Increasing possibility detecting key developing direct tissue proteomics especially any potential functional investigation. data will open door step imaging.

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