Proteomics in Diagnostic Pathology : Profiling and Imaging Proteins Directly in Tissue Sections

摘要: Direct tissue profiling and imaging mass spectrometry (MS) provide a molecular assessment of numerous expressed proteins within sample. MALDI MS (matrix-assisted laser desorption ionization) analysis thin sections results in the visualization 500 to 1000 individual protein signals weight range from 2000 over 200,000. These directly correlate with distribution specific region The systematic investigation section allows construction ion density maps, or images, for virtually every signal detected analysis. Ultimately, hundreds each at weight, may be obtained. To date, has been applied multiple diseased tissues, including human non-small cell lung tumors, gliomas, breast tumors. Interrogation resulting complex data sets using modern biocomputational tools resulted identification both disease-state patient-prognosis patterns. studies suggest that such proteomic information will become more important assessing disease progression, prognosis, drug efficacy. Molecular histology known some time its value clear field pathology. Imaging brings new dimension data, one focusing on phenotype. present article reviews state art technology complementarity traditional histopathological analyses.