Quantitative estimation of transmembrane ion transport in rat renal collecting duct principal cells.

摘要: Kidney collecting duct principal cells play a key role in regulated tubular reabsorption of water and sodium secretion potassium. The importance this function for the maintenance osmotic homeostasis whole organism motivates extensive study ion transport properties cells. We performed experimental measurements cell volume intracellular concentration rat renal from outer medulla (OMCD) used mathematical model describing transmembrane fluxes to analyze data. chloride concentrations ([Na+]in = 37.3 ± 3.3 mM, [Cl-]in 32.2 4.0 mM) OMCD were quantitatively estimated. Correspondence between experimentally measured physiological characteristics values permeability parameters was established. Plasma membrane permeabilities rates sodium, potassium ions estimated on basis substitution experiments predictions. In particular, calculated (PNa), (PK) (PCl) equal 3.2 × 10-6 cm/s, 1.0 10-5 cm/s 3.0 respectively. This approach sets grounds utilization quantify aids us understanding physiology adjustment excretion.