Vasopressin and hyperosmolality regulate NKCC1 expression in rat OMCD.
作者： Kimio Tomita , Shiho Wakamatsu , Hiroshi Nonoguchi , Mika Ikebe , Kenji Machida
DOI: 10.1038/HR.2009.52
关键词:
摘要: Secretory-type Na-K-2Cl cotransporter (NKCC1) is known to play roles in both acid and sodium excretion, more abundant dehydration. To determine the mechanisms by which dehydration stimulates NKCC1 expression, effects of vasopressin, oxytocin hyperosmolality on mRNA protein expressions outer medullary collecting duct (OMCD) rats were investigated using RT-competitive PCR western blot analysis. Microdissected OMCD was incubated isotonic or hypertonic solution, with AVP for 60 min at 37 degrees C. Hyperosmolality induced NaCl, mannitol raffinose increased expression 130-240% vitro. The stimulation NaCl highest 690 mosmol kg(-1) H(2)O gradually decreased higher osmolalities. incubation (10(-7) M) 100%. administration 4 days an osmotic mini-pump also 130%. In contrast, did not stimulate Chronic injection 36%. These data showed that vasopressin rat OMCD. It concluded regulated directly indirectly vasopressin.
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