Cell locomotion forces versus cell contraction forces for collagen lattice contraction: an in vitro model of wound contraction.

摘要: Cultured human dermal fibroblasts suspended in a rapidly polymerizing collagen matrix produce fibroblast-populated lattice. With time, this lattice will undergo reduction size referred to as contraction. During process, two distinct cell populations develop. At the periphery of lattice, highly oriented sheets cells, morphologically identifiable myofibroblasts, show cell-to-cell contacts and thick, actin-rich staining cytoplasmic stress fibers. It is proposed that these cells undergoing contraction multicellular contractile unit which reorients fibrils associated with them. The central region, fibroblasts, are randomly oriented, few faintly actin filaments. In contrast it working single units use locomotion forces reorient Using model, we sought determine mechanisms, or locomotion, responsible for force contracts lattices. Our experiments showed force, mechanism appears be related locomotion. This myofibroblast; where based upon contractions. Fibroblasts attempting move within reorganize surrounding fibrils; when can organized no further develop, occurs at first, longer participate dynamic aspects