Purification of O-specific polysaccharide from lipopolysaccharide produced by Salmonella enterica serovar Paratyphi A.

摘要: The O specific polysaccharide (OSP) of the lipopolysaccharide (LPS) Salmonella enterica serovar Paratyphi A is a protective antigen and target for vaccine development. LPS major constituent outer membrane S. with OSP exposed on surface, in addition to cell associated large amount free was present fermentation broth. purification method developed take advantage both sources maximize recovery OSP. After bacterial cells were concentrated washed, permeate containing processed separately from cells. washed 100kD ultrafiltration remove low molecular weight impurities. then detoxified by separation lipid using acid hydrolysis at 100°C, precipitated removed 0.2μm filtration. Contaminants precipitation presence sodium deoxycholate. 1M NaCl water 10kD sterile filtered through filter. treated remaining cells, debris precipitate centrifugation. filtrate same way as described above LPS. combined yield purified plus greater than 880mg/L culture yields amounts OSP, scalable compatible cGMP so would be readily transferrable developing country manufacturers cost production against A.