Assembly of the Brainstem Cochlear Nuclear Complex Is Revealed by Intersectional and Subtractive Genetic Fate Maps

摘要: The cochlear nuclear complex (CN) is the entry point for central auditory processing. Although constituent neurons have been studied physiologically, their embryological origins and molecular profiles remain obscure. Applying intersectional subtractive genetic fate mapping approaches, we show that this develops modularly from genetically separable progenitor populations arrayed as rostrocaudal microdomains within outside hindbrain (lower) rhombic lip (LRL). dorsal CN subdivision, structurally topographically similar to cerebellum, arises unexpectedly caudal noncontiguous cerebellar primordium; ventral subdivisions arise more rostral LRL. Magnocellular regions receive contributions LRL coaxial non-lip progenitors; contrastingly, ensheathing granule cells derive principally Also LRL-derived molecularly are precerebellar mossy fiber neurons; surprisingly, these ostensibly intertwined adjacent but segregated migratory streams. Together, findings provide new platforms investigating development evolution of systems.