Cellular distribution of COT1 kinase in Neurospora crassa.

摘要: The Neurospora crassa cot-1 gene encodes a Ser/Thr protein kinase, which is involved in hyphal elongation. Many vacuoles, abnormally shaped mitochondria, and nuclei, along with differences the structure of cell wall septa, were observed hyphae mutant shortly after shift to restrictive temperature. Immunolocalization experiments indicated that COT1 was associated cytoplasmic membrane; also detected cytoplasm. membrane-associated absent from when shifted temperature, as lower molecular weight isoform COT1. We propose may be several cellular processes, spatial temporal regulation activity involves trafficking kinase within fungal its possible interaction additional proteins.