Dielectric Study of the Physical State of Electrolytes and Water Within Bacillus cereus Spores

摘要: Dielectric measurements revealed that dormant spores of Bacillus cereus have extremely low conductivities at high frequencies (50 MHz) and so must contain remarkably concentrations mobile ions both within the core in surrounding integuments. Activation, germination, outgrowth were all accompanied by increases conductivity cells their suspending medium, this result indicated intracellular electrolytes had become ionized leaked from spores. High-frequency dielectric constants consistent with normal states for cell water. These values increased during successive stages development spore to vegetative bacillus, they could be directly related water content. In all, results refuted a model involving freely mobile, supported electrostatically bound electrolytes.