Diagnosis of BK Viral Nephropathy in the Renal Allograft Biopsy: Role of Fluorescence in Situ Hybridization

摘要: Early recognition of BK viral nephropathy is essential for successful management. Our aim in this study was to evaluate a novel fluorescence situ hybridization (FISH) assay detection virus renal transplant biopsies the context standard methods. Renal allograft (n = 108) were analyzed via HE 24 108 showed discrepancy ≥1 testing modalities. Comparison H&E, IHC, and FISH no statistical difference virus. However, performing comparisons between different tissue-based assays plasma or urine real-time PCR results significant improvement by over H&E (P 0.02) but not IHC 0.07). This FISH-based approach identification biopsy tissue mirrored superior performance inclusions H&E. Therefore, use setting useful sensitive method could be adopted any laboratory that currently performs analysis.