Isotachophoresis as an on-line concentration pretreatment technique in capillary electrophoresis.

摘要: One of the major disadvantages capillary electrophoresis (CE) is its limited loadability. Therefore, on-line coupling isotachophoresis (ITP) and CE was studied with regard to potential for improvement minimum concentration that can be measured by CE. Based on concentrating separating power ITP, detection limits could lowered at least two orders magnitude. Especially biological samples containing proteins, it appeared in non-treated capillaries electromigration characteristics are hardly influenced when isotachophoretic pretreatment applied. The ITP-CE illustrated analysis o-phthaldialdehyde fluorescein isothiocyanate derivatives a number amino acids.