Mevalonate kinase is a cytosolic enzyme in humans

摘要: In the past decade several reports have appeared which suggest that peroxisomes play a central role in isoprenoid/cholesterol biosynthesis. These suggestions were based primarily on reported finding of enzymes presqualene segment biosynthetic pathway peroxisomes. More recently, however, conflicting results been raising doubt about postulated isoprenoid biosynthesis, at least humans. this study we studied subcellular localisation human mevalonate kinase (MK) using variety biochemical and microscopical techniques. include conventional fractionation studies, digitonin permeabilisation immunofluorescence microscopy immunocytochemistry. We exclusively found cytosolic both endogenous MK (human fibroblasts, liver HEK293 cells) overexpressed cells CV1 cells). No indication peroxisomal was obtained. Our do not support for