Transforming growth factor-β3 protection of epithelial cells from cycle-selective chemotherapy in vitro

摘要: Abstract The transforming growth factor-β (TGF-β) family of regulatory factors can reversibly arrest cell division in the G 1 phase cycle. Previously, TGF-β3 was shown to protect epithelial cells and hematopoietic from cytotoxic damage vitro vivo , reduce severity duration oral mucositis induced by 5-fluorouracil (5-FU) . In present study, we tested whether a range chemotherapy drugs with differing mechanisms action, using CCL64 line as model system. We report that preincubation for 24 hr resulted enhanced clonogenicity following exposure vinblastine, vincristine, etoposide, taxol, ara-C, methotrexate, or 5-FU. Protection measured colony-forming assays, which demonstrated protected could re-enter cycle undergo multiple rounds division. At high drug concentrations, absolute colony counts were increased cultures prearrested TGF-β3, compared proliferating control cultures. effects reduced cisplatin doxorubicin, are toxic throughout Thus, effectively cytotoxicity anticancer act predominantly S M