Adenoviral (full-length) Apo2L/TRAIL gene transfer is an ineffective treatment strategy for malignant glioma.
作者： Ulrike Naumann , Robert Waltereit , Jörg B. Schulz , Michael Weller
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摘要: Death ligand-mediated apoptosis is a promising strategy of gene therapy for human malignant glioma. We here report that the infection glioma cell lines with an adenoviral vector encoding full length Apo2 ligand/tumor necrosis factor-related apoptosis-inducing ligand (Ad-Apo2L/TRAIL) results in strong Apo2L/TRAIL transgene expression and release full-length into culture medium. However, Ad-Apo2L/TRAIL poor inducer death, even presence inhibitors protein synthesis, which are sensitive to soluble recombinant His-tagged (amino acids 114-281). Moreover, transfer inhibits Apo2L/TRAIL-induced apoptosis, strongly suggesting adenovirally encoded not suitable molecule cancer therapy. This study has important implications future development therapeutic strategies aiming at death receptor activation refractory cancers such as
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