Polyethylenimine-grafted copolymer of poly(l-lysine) and poly(ethylene glycol) for gene delivery.

摘要: A major challenge in gene therapy is the development of effective delivery vectors with low toxicity. In present study, linear poly(ethylenimine) (lPEI) molecular weight was grafted onto block copolymer (PPL) poly(l-lysine) (PLL) and poly(ethylene glycol)(PEG), yielding a ternary PEG-b-PLL-g-lPEI (PPI) for delivery. such design, PLL, lPEI PEG blocks were expected to render vector biodegradability, proton buffering capacity, cationic toxicity potentially long circulation vivo, respectively. Given proper control composition, copolymers demonstrated lower cytotoxicity, ability condense pDNA mediate transfection various cell lines. With folate as an exemplary targeting ligand, FA-PPI/pDNA complex showed much higher transgene activity than its nontargeting counterpart both reporter therapeutic genes receptor(FR)-positive cells. FA-PPI mediated TNF-related apoptosis-inducing ligand (TRAIL) human hepatoma Bel 7402 cells, leading apoptosis great suppression viability. Our results indicate that might be promising combining high efficiency.