Toxicological effects of benzo(a)pyrene, DDT and their mixture on the green mussel Perna viridis revealed by proteomic and metabolomic approaches.
作者： Qinqin Song , Hao Chen , Yuhu Li , Hailong Zhou , Qian Han
DOI: 10.1016/J.CHEMOSPHERE.2015.08.029
关键词:
摘要: Benzo(a)pyrene (BaP) and dichlorodiphenyltrichloroethane (DDT) are persistent organic pollutants environmental estrogens (EEs) with known toxicity towards the green mussel, Perna viridis. In this study, toxic effects of BaP (10 µg/L) DDT their mixture were assessed in mussel gills proteomic metabolomic approaches. Metabolic responses indicated that mainly caused disturbance osmotic regulation by significantly decrease branched chain amino acids, dimethylamine dimethylglycine male mussels after exposure for 7 days. energy metabolism differential alteration betaine, dimethylamine, dimethylglycine, succinate mussels. However, didn't show obvious metabolite changes. Proteomic analysis showed different protein expression profiles between treatment groups, which demonstrated BaP, may have modes action. revealed induced cell apoptosis, digestion mussels, whereas altered proteins associated oxidative stress, cytoskeleton structure, metabolism. affected related to biosynthesis modification, metabolism, growth apoptosis.
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sci-hub.se 参考文章(98)
John C. Lindon, Jeremy K. Nicholson, Jeremy R. Everett, NMR Spectroscopy of Biofluids Annual reports on NMR spectroscopy. ,vol. 38, pp. 1- 88 ,(1999) , 10.1016/S0066-4103(08)60035-6
Kembra L. Howdeshell, Andrew K. Hotchkiss, Kristina A. Thayer, John G. Vandenbergh, Frederick S. vom Saal, Exposure to bisphenol A advances puberty Nature. ,vol. 401, pp. 763- 764 ,(1999) , 10.1038/44517
F Kargin, HY Cogun, None, Metal interactions during accumulation and elimination of zinc and cadmium in tissues of the freshwater fish Tilapia nilotica. Bulletin of Environmental Contamination and Toxicology. ,vol. 63, pp. 511- 519 ,(1999) , 10.1007/S001289901010
PH O'Farrell, High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry. ,vol. 250, pp. 4007- 4021 ,(1975) , 10.1016/S0021-9258(19)41496-8
Hadassa Degani, Dalia Seger, Dalia Rivenson-Segal, Rachel Katz-Brull, Edna Rushkin, Metabolic Markers of Breast Cancer Enhanced Choline Metabolism and Reduced Choline-Ether-Phospholipid Synthesis Cancer Research. ,vol. 62, pp. 1966- 1970 ,(2002)
Przemysław WOJTASZEK, Oxidative burst: an early plant response to pathogen infection Biochemical Journal. ,vol. 322, pp. 681- 692 ,(1997) , 10.1042/BJ3220681
S Wera, B A Hemmings, Serine/threonine protein phosphatases Biochemical Journal. ,vol. 311, pp. 17- 29 ,(1995) , 10.1042/BJ3110017
A. Polder, M.B. Müller, J.L. Lyche, R.H. Mdegela, H.E. Nonga, F.P. Mabiki, T.J. Mbise, J.U. Skaare, M. Sandvik, E. Skjerve, E. Lie, Levels and patterns of persistent organic pollutants (POPs) in tilapia (Oreochromis sp.) from four different lakes in Tanzania: geographical differences and implications for human health. Science of The Total Environment. ,vol. 488, pp. 252- 260 ,(2014) , 10.1016/J.SCITOTENV.2014.04.085
Chenglong Ji, Huifeng Wu, Lei Wei, Jianmin Zhao, Qing Wang, Hongjian Lu, Responses of Mytilus galloprovincialis to bacterial challenges by metabolomics and proteomics. Fish & Shellfish Immunology. ,vol. 35, pp. 489- 498 ,(2013) , 10.1016/J.FSI.2013.05.009
Jia Xin, Xiang Liu, Wei Liu, Lu Jiang, Jihua Wang, Jia Niu, Production and use of DDT containing antifouling paint resulted in high DDTs residue in three paint factory sites and two shipyard sites, China. Chemosphere. ,vol. 84, pp. 342- 347 ,(2011) , 10.1016/J.CHEMOSPHERE.2011.04.005