Genotyping and phylogenetic analysis of bovine viral diarrhea virus isolates from BVDV infected alpacas in North America.

摘要: Over a three-year period, 2004-2007, greater than 12,000 alpacas in the United States were screened by real-time RT-PCR to identify persistently infected (PI) with bovine viral diarrhea virus (BVDV). A total of 46 BVD viruses isolated from PI or diagnostic samples alpacas. Forty-three US alpaca BVDV isolates and 3 Canadian analyzed comparison nucleotide sequences two genomic regions, 5'-UTR N(pro) gene determine their genetic relatedness. All 8 different states Canada genotype 1b > =99% nt identity 290-base region exception one isolate. In contrast, 21 collected during same period grouped into typical genotypes, 1a, 1b, 2, respectively. Forty five formed distinctive cluster separated closely related phylogenetic analysis region. Comparison 504-base 32 also assigned them all type similar fashion as observed The results suggest that unique genotypes may be maintained population even though camelids are susceptible infection other genotypes. Further studies needed address why predominantly how evolved infect