Rethinking Fundamentals of Enzyme Action

摘要: Despite certain limitations, investigators continue to gainfully employ concepts rooted in steady-state kinetics efforts draw mechanistically relevant inferences about enzyme catalysis. By reconsidering kinetic behavior, this review develops ideas that allow one arrive at the following new definitions: (a) V/K, ratio of maximal initial velocity divided by Michaelis-Menten constant, is apparent rate constant for capture substrate into complexes are destined yield product(s) some later point time; (b) V release from captured form free product(s); and (c) K constants capture. The physiologic significance V/K also explored illuminate aspects antibiotic resistance, concept "perfection" catalysis, catalytic proficiency. conceptual basis congruent thermodynamic cycles considered an attempt achieve unambiguous way comparing enzyme-catalyzed reaction with its uncatalyzed reference reaction. Such promise a deeper understanding origins power, as it relates stabilization reactant ground state, transition reciprocal stabilizations states.