Illustrating Enzyme Inhibition Using Gibbs Energy Profiles

摘要: Gibbs energy profiles have great utility as teaching and learning tools because they present students with a visual representation of the changes that occur during enzyme catalysis. Unfortunately, most textbooks divorce discussions traditional kinetic topics, such inhibition, from these same topics in terms profiles. Examination values apparent parameters KSapp, kcatapp, (kcat/KM)app response to various modes inhibition may be informative when presented combination Herein, symbolism standard is utilized derive expressions for associated describe their behavior presence either competitive, uncompetitive, noncompetitive, or linear mixed-type inhibitor under rapid equilibrium conditions. The approach intuitive complementary deriva...