Specificity and degeneracy in peptide binding to HLA-B7-like class I molecules.

摘要: The HLA-B7-like binding supertype includes several different HLA-B molecules. Herein, the primary and secondary anchor specificities of five most common molecules (B*0702, B*3501, B51, B*5301, B*5401) were defined by use molecular assays, analogue peptides, large sets peptides corresponding to naturally occurring sequences. All B7-like analyzed preferentially bound 9-mers, with a stringent requirement for proline in position 2, while variety hydrophobic or aromatic residues well tolerated at C-terminal position. Although an allele-specific fashion, approximately 20% binders identified degenerate least three affinities 500 nM less. It was also noted that, general, that bind high affinity any given one molecule frequently capable binding. Prominent roles anchors positions 1 3 observed molecules, motifs utilized derive supermotif. validity this supermotif tested blind prediction set. Finally, general strategy rationally engineering peptide analogues sequences greatly increased degeneracy. Such engineered may be significant utility development peptide-based vaccines against chronic viral diseases cancer.