Characterization of the peptide binding motif of a rhesus MHC class I molecule (Mamu-A*01) that binds an immunodominant CTL epitope from simian immunodeficiency virus.

摘要: The majority of immunogenic CTL epitopes bind to MHC class I molecules with high affinity. However, peptides longer or shorter than the optimal epitope rarely Therefore, identification from pathogens may ultimately be critical for inducing strong responses and developing epitope-based vaccines. SIV-infected rhesus macaque is an excellent animal model HIV infection humans. Although a number have been mapped in macaques, not well defined, their anchor residues are unknown. We now defined SIV gag restricted by molecule Mamu-A*01 general peptide binding motif this that characterized dominant position 3 (proline). used elution sequencing, assays, bulk clonal assays demonstrate Mamu-A*01-restricted was CTPYDINQM181–189. unique it found at frequency all Mamu-A*01-positive macaques studied date develop immunodominant gag-specific response molecule. Identification will variety studies designed induce CD8+ specific macaque.