Diphosphorylation of platelet myosin ex vivo in the initial phase of activation by thrombin.
作者： Kazuyuki Itoh , Taeko Hara , Fumiko Yamada , Nobuhiko Shibata
DOI: 10.1016/0167-4889(92)90084-O
关键词:
摘要: Abstract We prepared anti-platelet 20-kDa myosin light chain (MLC-20) antibody and demonstrated diphosphorylation of MLC-20 in platelets ex vivo the initial phase activation by thrombin. Our results are as follows. (1) By Western blotting, using anti-MLC-20 antibody, both mono- diphosphorylated were seen aggregation The peak was later than that monophosphorylation degree reduced process aggregation. (2) ML-7 (a synthetic inhibitor MLCK) inhibited also blocked thrombin-activated platelets. However, H-7 (an protein kinase C) had little effect on either (di)phosphorylation or (3) Arg-Gly-Asp-Ser (RGDS) peptide, a anti-adhesive dose-dependent manner (100–200 μM). it platelet stimulated From these results, we conclude MLCK play role thrombin-stimulated precedes secondary signal mediated GPIIb/IIIa.
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