Essential role of myosin light chain kinase in the mechanism for MgATP- dependent priming of exocytosis in adrenal chromaffin cells
作者： K Kumakura , K Sasaki , T Sakurai , M Ohara-Imaizumi , H Misonou
DOI: 10.1523/JNEUROSCI.14-12-07695.1994
关键词:
摘要: Ca(2+)-induced exocytosis in chromaffin cells now seems to consist of at least two distinct steps:MgATP-dependent Ca(2+)-dependent priming the secretory apparatus, and MgATP-independent step that triggers (Bittner Holz, 1992). Recently we found a specific inhibitor myosin light chain kinase (MLCK), wortmannin, inhibits catecholamine release from digitonin-permeabilized cells, suggesting an implication MLCK mechanisms (Imaizumi et al., 1992b). To elucidate further mechanism exocytosis, studied effects wortmannin peptide (SM-1) corresponding pseudosubstrate domain on MgATP-dependent cells. permeabilized presence MgATP was inhibited by both SM-1 wortmannin. Inhibitory effect rate induced 10 microM Ca2+ much prominent later phase (1-10 min), although initial also decreased. strongly ATP-dependent without affecting ATP-independent all. In addition, underlies remarkably reduced SM-1. These results suggest plays essential role
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