Use of fish trypsin immobilized onto magnetic-chitosan composite as a new tool to detect antinutrients in aquafeeds.

摘要: The unplanned inclusion of antinutrients in fish food affects many biological processes, such as digestibility amino acids and diet conversion, resulting undesirable effects on body growth. Thus, the objective this research was to propose use immobilized proteases detection protease inhibitors, one most important antinutrients. In order evaluate antinutritional factors through trypsin, enzyme incubated with eight diets developed for commercial fish, residual activity measured. Comparatively, tilapia trypsin showed an inhibition (protease inhibitors), present studied diets, up 48% greater than porcine magnetic chitosan. it is possible suggest derivatives containing specific target organism that reduce animal's digestive capacity negatively influence their growth during husbandry.