Atractylodes macrocephala Koidz stimulates intestinal epithelial cell migration through a polyamine dependent mechanism

摘要: Abstract Ethnopharmacological relevance Atractylodes macrocephala Koidz (AMK), a valuable traditional Chinese herbal medicine, has been widely used in clinical practice for treating patients with disorders of the digestive system. AMK shown noteworthy promoting effect on improving gastrointestinal function and immunity, which might represent promising candidate treatment intestinal mucosa injury. The aim this study was to investigate efficacy mucosal restitution underlying mechanisms via epithelial (IEC-6) cell migration model. Materials methods A model IEC-6 cells induced by single-edge razor blade along diameter layers six-well polystyrene plates. After wounding, were grown control cultures containing spermidine (5 μM, SPD, reference drug), alpha-difluoromethylornithine (2.5 mM, DFMO, polyamine inhibitor), (50, 100, 200 mg/L), DFMO plus SPD 12 h. polyamines content detected high-performance liquid chromatography (HPLC) pre-column derivatization. Rho mRNAs expression levels assessed Q-RT-PCR. non-muscle myosin II proteins analyzed Western blot. formation distribution stress fibers monitored immunostaining techniques using specific antibodies observed confocal microscopy. Cell assay carried out inverted microscope Image-Pro Plus software. All these indexes evaluate effectiveness AMK. Results (1) Treatment caused significant increases cellular levels, as compared group. Furthermore, exposure increased protein fibers, resulted an acceleration cells. (2) Depletion decrease expression, distribution, thereby inhibiting migration. not only reversed inhibitory effects content, but also restored levels. Conclusions results obtained from revealed that significantly stimulates through dependent mechanism, could accelerate healing These findings suggest potential value curing diseases characterized injury ineffective repair practice.