An enzymatic assay for determining free and total cholesterol in tissue.
作者： J L De Hoff , L M Davidson , D Kritchevsky
DOI: 10.1093/CLINCHEM/24.3.433
关键词:
摘要: We describe a method for determining free and total cholesterol in extracts of rat-liver lipids by use esterase/cholesterol oxidase enzymatic kit (Boehringer Mannheim Corp). The are solubilized (as micelles) directly into the aqueous reagent mixture. Results correlate well with those obtained an acid/ferric chloride assay digitonin precipitates extracts. tissue offers advantages simplicity over previously used methods.
oup.com
aaccjnls.org参考文章(7)
Warren M. Sperry, Merrill Webb, A revision of the Schoenheimer-Sperry method for cholesterol determination Journal of Biological Chemistry. ,vol. 187, pp. 97- 106 ,(1950) , 10.1016/S0021-9258(19)50934-6
B Zak, Cholesterol methodologies: a review. Clinical Chemistry. ,vol. 23, pp. 1201- 1214 ,(1977) , 10.1093/CLINCHEM/23.7.1201
C.M. Van Gent, H.A. Van Der Voort, A.M. De Bruyn, F. Klein, Cholesterol determinations. a comparative study of methods with special reference to enzymatic procedures Clinica Chimica Acta. ,vol. 75, pp. 243- 251 ,(1977) , 10.1016/0009-8981(77)90195-4
Ari Helenius, Kai Simons, Solubilization of membranes by detergents Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes. ,vol. 415, pp. 29- 79 ,(1975) , 10.1016/0304-4157(75)90016-7
Jordi Folch, M. Lees, G.H. Sloane Stanley, A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES Journal of Biological Chemistry. ,vol. 226, pp. 497- 509 ,(1957) , 10.1016/S0021-9258(18)64849-5
P Roeschlau, W Gruber, E Bernt, Enzymatic determination of total cholesterol in serum. Clinical Chemistry and Laboratory Medicine. ,vol. 12, pp. 226- 226 ,(1974)
B. Zak, R. C. Dickenman, E. G. White, H. Burnett, P. J. Cherney, Rapid estimation of free and total cholesterol. American Journal of Clinical Pathology. ,vol. 24, pp. 1307- 1315 ,(1954) , 10.1093/AJCP/24.11_TS.1307