Regulatory effects of IL-12 and IL-18 on Onchocerca volvulus- and Entamoeba histolytica-specific cellular reactivity and cytokine profiles.

摘要: Abstract In the present study, cytokines interleukin (IL)-12 and IL-18 were evaluated for their capacity to modulate re-direct in vitro parasite antigen-specific cellular responsiveness patients exposed Onchocerca volvulus Entamoeba histolytica infection. We found that was highly capable of reducing antigen-induced IL-10 production by PBMC. contrast, addition or neutralization IL-12, also combination with interferon-gamma-inducible chemokine IP-10 did not affect production. Interestingly, highest levels measured when both neutralized. Although having no effect on IL-10, IL-12 strongly promoted spontaneous antigen-driven IFN-gamma PBMC, whereas only moderately affecting release PBMC re-stimulated E. histolytica- O. volvulus-specific antigens. Both diminished IL-13, a synergistic observed combined. Likewise, enhanced IL-13 production, but further increase observed, anti-IL-12 anti-IL-18 used together. This study disclosed will significantly down-regulate parasite-specific induced inhibited from humans histolytica. Such selective immune-regulatory may comprise an important tool polarized cytokine responses towards balanced Th1/Th2 profile, which prevent pathology promote immunity against helminth protozoan infections.