Rapid Assay of Binding of Tumor-Promoting Phorbol Esters to Protein Kinase C

摘要: Protein kinase C is generally accepted to be a receptor protein of tumor-promoting phorbol esters. The binding [3H]phorbol-12,13-dibutyrate can assayed by rapid filtration procedure using glass-fiber filter that has been treated with cationic polymer, polyethylenimine. ester specifically binds the only in presence phosphatidylserine and calcium. Non-specific less than 10%, at most, total binding. linear respect concentration C, dependent on concentrations saturative manner, inhibited diacylglycerol (an endogenous activator kinase).