Protein kinase C activity can desensitize the gonadotropin-responsive adenylate cyclase in Leydig tumor cells. But hCG-induced desensitization does not involve protein kinase C activation.
作者： Y Inoue , R V Rebois
DOI: 10.1016/S0021-9258(18)81820-8
关键词:
摘要: The murine Leydig tumor cell line, MLTC-1, contains a gonadotropin receptor-coupled adenylate cyclase. Although the binding of human choriogonadotropin (hCG) initially causes cells to accumulate cAMP, in time, response hCG is attenuated by desensitization. Treating intact with promoter, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), or diacylglycerol also desensitization response. These compounds are activators calcium/phospholipid-dependent protein kinase (PKC). MLTC-1 TPA dioctanoylglycerol increased portion PKC membrane fraction. This phenomenon associated activation PKC. isolated membranes purified desensitize Thus, caused probably mediated normally activated when phosphoinositides metabolized and inositol phosphates. There was no significant accumulation phosphates were treated hCG. did not increase However, could membranes, but not. We conclude that although C activity can response, does cause activating implications this observation discussed.
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