A native 170,000 epidermal growth factor receptor-kinase complex from shed plasma membrane vesicles.
作者： S. Cohen , H. Ushiro , C. Stoscheck , M. Chinkers
DOI: 10.1016/S0021-9258(19)68224-4
关键词:
摘要: A method is presented for the preparation of a "native" epidermal growth factor (EGF) receptor-kinase complex molecular weight 170,000 from A-431 cells. Although this receptor capable binding EGF, noncovalently, in quantities similar to previously isolated 150,000 (Cohen, S., Carpenter, G., and King, L., Jr. (1980) J. Biol. Chem. 255, 4834-4842), has 5 10 times intrinsic kinase activity (autophosphorylation). However, toward other proteins lower than that preparation. Both activities are enhanced by EGF. The also forming covalent linkages 125I-EGF, each precipitated antisera directed against protein. We suggest protein proteolytic degradation product EGF-enhanced remains associated with 125I-EGF-binding following EGF affinity chromatography, electrophoresis nondenaturing gels, or immunoprecipitation sodium dodecyl sulfate (SDS) gel-purified These results indicate receptor, kinase, substrate domains linked, possibly covalently.
sci-hub.st 参考文章(32)
D Thom, A J Powell, C W Lloyd, D A Rees, Rapid isolation of plasma membranes in high yield from cultured fibroblasts Biochemical Journal. ,vol. 168, pp. 187- 194 ,(1977) , 10.1042/BJ1680187
C. Richard Savage, Stanley Cohen, Epidermal Growth Factor and a New Derivative Journal of Biological Chemistry. ,vol. 247, pp. 7609- 7611 ,(1972) , 10.1016/S0021-9258(19)44568-7
L King, S Cohen, G Carpenter, Rapid enhancement of protein phosphorylation in A-431 cell membrane preparations by epidermal growth factor. Journal of Biological Chemistry. ,vol. 254, pp. 4884- 4891 ,(1979) , 10.1016/S0021-9258(17)30094-7
H. Ushiro, S. Cohen, Identification of phosphotyrosine as a product of epidermal growth factor-activated protein kinase in A-431 cell membranes. Journal of Biological Chemistry. ,vol. 255, pp. 8363- 8365 ,(1980) , 10.1016/S0021-9258(18)43497-7
Frederic M. Richards, Paul J. Vithayathil, The Preparation of Subtilisin-modified Ribonuclease and the Separation of the Peptide and Protein Components Journal of Biological Chemistry. ,vol. 234, pp. 1459- 1465 ,(1959) , 10.1016/S0021-9258(18)70031-8
S. Cohen, G. Carpenter, L. King, Epidermal growth factor-receptor-protein kinase interactions. Co-purification of receptor and epidermal growth factor-enhanced phosphorylation activity. Journal of Biological Chemistry. ,vol. 255, pp. 4834- 4842 ,(1980) , 10.1016/S0021-9258(19)85573-4
M. Spector, S. O'Neal, E. Racker, Regulation of phosphorylation of the beta-subunit of th Ehrlich ascites tumor Na+K+-ATPase by a protein kinase cascade. Journal of Biological Chemistry. ,vol. 256, pp. 4219- 4227 ,(1981) , 10.1016/S0021-9258(19)69421-4
George Scatchard, The Attractions of Proteins for Small Molecules and Ions Annals of the New York Academy of Sciences. ,vol. 51, pp. 660- 672 ,(1949) , 10.1111/J.1749-6632.1949.TB27297.X
JOFFRE B. BAKER, ROBERT L. SIMMER, KEVIN C. GLENN, DENNIS D. CUNNINGHAM, Thrombin and epidermal growth factor become linked to cell surface receptors during mitogenic stimulation. Nature. ,vol. 278, pp. 743- 745 ,(1979) , 10.1038/278743A0
Michael Chinkers, Stanley Cohen, Purified EGF receptor–kinase interacts specifically with antibodies to Rous sarcoma virus transforming protein Nature. ,vol. 290, pp. 516- 519 ,(1981) , 10.1038/290516A0