A complement-mediated rat xenotransfusion model of platelet refractoriness.
作者： Adrianne I. Enos , Pamela S. Hair , Neel K. Krishna , Kenji M. Cunnion
DOI: 10.1016/J.MOLIMM.2020.05.008
关键词:
摘要: Abstract Background Platelet refractoriness remains a challenging clinical dilemma although significant advancements have been made in identifying human leukocyte antigen (HLA) matched or HLA compatible units. Antiplatelet antibodies are the major risk factor for immune-mediated platelet refractoriness, yet role of antibody-initiated complement-mediated destruction poorly understood. Study Design and Methods Human opsonization killing platelets was assayed ex vivo using antibody-sensitized incubated with complement-sufficient sera. A new animal model utilizing Wistar rats transfused is described. Results sensitized anti-platelet were rapidly opsonized iC3b upon incubation This could be completely blocked classical pathway complement inhibitor, PA-dPEG24. Complement activation decreased viability, which also reversible inhibitor rat developed that demonstrated some removal from blood stream mediated. Conclusions initiated by leads to viability. adds tool elucidating mechanisms refractoriness.
sci-hub.st 参考文章(23)
Julia A. Sharp, Pamela S. Hair, Haree K. Pallera, Parvathi S. Kumar, Clifford T. Mauriello, Julius O. Nyalwidhe, Cody A. Phelps, Dalnam Park, Nicole M. Thielens, Stephen M. Pascal, Waldon Chen, Diane M. Duffy, Frank A. Lattanzio, Kenji M. Cunnion, Neel K. Krishna, Peptide Inhibitor of Complement C1 (PIC1) Rapidly Inhibits Complement Activation after Intravascular Injection in Rats PLOS ONE. ,vol. 10, pp. e0132446- ,(2015) , 10.1371/JOURNAL.PONE.0132446
Parvathi S. Kumar, Clifford T. Mauriello, Pamela S. Hair, Nicholas S. Rister, Courtney Lawrence, Reem H. Raafat, Kenji M. Cunnion, Glucose-based dialysis fluids inhibit innate defense against Staphylococcus aureus. Molecular Immunology. ,vol. 67, pp. 575- 583 ,(2015) , 10.1016/J.MOLIMM.2015.07.017
Simon J. Stanworth, Cristina Navarrete, Lise Estcourt, Judith Marsh, Platelet refractoriness--practical approaches and ongoing dilemmas in patient management. British Journal of Haematology. ,vol. 171, pp. 297- 305 ,(2015) , 10.1111/BJH.13597
JB Carlin, LW Doyle, Statistics for clinicians: 4: Basic concepts of statistical reasoning: hypothesis tests and the t-test. Journal of Paediatrics and Child Health. ,vol. 37, pp. 72- 77 ,(2001) , 10.1046/J.1440-1754.2001.00634.X
Shinobu Tanaka, Takakazu Suzuki, Kusuya Nishioka, Assay of classical and alternative pathway activities of murine complement using antibody-sensitized rabbit erythrocytes Journal of Immunological Methods. ,vol. 86, pp. 161- 170 ,(1986) , 10.1016/0022-1759(86)90448-5
M.L. Rand, H. Wang, M. Mody, I. Chu, I. Treutiger, A. Nguyen, M.A. Packham, J. Freedman, Concurrent measurement of the survival of two populations of rabbit platelets labeled with either two PKH lipophilic dyes or two concentrations of biotin Cytometry. ,vol. 47, pp. 111- 117 ,(2002) , 10.1002/CYTO.10055
Katerina Pavenski, John Freedman, J. W. Semple, HLA alloimmunization against platelet transfusions: pathophysiology, significance, prevention and management. Tissue Antigens. ,vol. 79, pp. 237- 245 ,(2012) , 10.1111/J.1399-0039.2012.01852.X
Pamela S. Hair, Jenny Q. Gronemus, Katrina B. Crawford, Veena P. Salvi, Kenji M. Cunnion, Nicole M. Thielens, Gérard J. Arlaud, Nenoo Rawal, Neel K. Krishna, Human astrovirus coat protein binds C1q and MBL and inhibits the classical and lectin pathways of complement activation. Molecular Immunology. ,vol. 47, pp. 792- 798 ,(2009) , 10.1016/J.MOLIMM.2009.10.006
Sherrill J Slichter, Kathryn Davis, Helen Enright, Hayden Braine, Terry Gernsheimer, Kuo-Jang Kao, Thomas Kickler, Edward Lee, Janice McFarland, Jeffrey McCullough, Glenn Rodey, Charles A Schiffer, Robert Woodson, Factors affecting posttransfusion platelet increments, platelet refractoriness, and platelet transfusion intervals in thrombocytopenic patients Blood. ,vol. 105, pp. 4106- 4114 ,(2005) , 10.1182/BLOOD-2003-08-2724
Jenny Q. Gronemus, Pamela S. Hair, Katrina B. Crawford, Julius O. Nyalwidhe, Kenji M. Cunnion, Neel K. Krishna, Potent inhibition of the classical pathway of complement by a novel C1q-binding peptide derived from the human astrovirus coat protein Molecular Immunology. ,vol. 48, pp. 305- 313 ,(2010) , 10.1016/J.MOLIMM.2010.07.012