Derivation of monoclonal antibodies to human somatomedin C/insulin-like growth factor I.

摘要: Summary Somatomedin C, also called insulin-like growth factor I (Sm-C/IGF-I), is a highly conserved polypeptide required for the proliferation of many cell types. Since several attempts in our laboratory to recover monoclonal antibody-secreting hybrids this peptide by direct fusion hyperimmunized splenocytes with myeloma cells had been unsuccessful, we modified approach coculturing BALB/c and small amount antigen 5 days prior P3X63Ag.8.653 line. Of 88 microcultures at risk, specific antibody was detected 24. Two clones were expanded ascites fluid characterized as isotype, affinity, specificity. Both IgG 1,κ bound human Sm-C/IGF-I affinity constants 1.09 1.01 × 10 liter/mol, respectively. quite inconsequential binding II, multiplication-stimulating activity, any chymotryptic fragments Sm-C/IGF-I, insulin preparations, hGH, hTSH, mEGF, or mouse albumin. In vitro boosting after primary vivo immunization appears provide monoclones an IgF isotype contrast immunization, which reportedly favors IgM isotype. The antibodies produced study have proved be extraordinary useful defining physiologic role Sm-I/IGF-I immunoneutralization techniques purification chromatography.