Rat C6 Glial Cells Synthesize Insulin-Like Growth Factor I (IGF-I) and Express IGF-I Receptors and IGF-II/ Mannose 6-Phosphate Receptors*
作者： WIELAND KIESS , LILLY LEE , DALE E. GRAHAM , LAWRENCE GREENSTEIN , LUCY Y.-H TSENG
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摘要: We have used the rat C6 glial cell line as a model system to study role of insulin-like growth factors (IGF) in neuroglial cells central nervous (CNS). Northern blot analysis RNA demonstrated presence IGF-I mRNA and undetectable IGF-II mRNA. IGF-binding protein (s), but not IGF-II, were detected cell-conditioned medium. The level was 1-4 ng/ml conditioned medium based on human standard. immunoreactive inhibited [125I] binding receptor chick embryo fibroblasts stimulated [3H] thymidine incorporation into fibroblast DNA. Competitive affinity cross-linking experiments using [125]IGF-I receptors (type I) IGF-II/mannose 6-phosphate II) membranes. An immunoglobulin (no. 3637) directed against blocked degradation [125I]IGF-II added cells, presumably ...
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