Plant adpglucose pyrophosphatase, method for the production thereof, its use in the production of assay devices and for obtaining transgenic plants

作者: Milagros Rodriguez Lopez , Francisco José Muñoz Perez , Javier Pozueta Romero , Edurne Baroja Fernandez , Aitor Zandueta Criado

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摘要: Plant ADPglusose pyrophosphatases, process for production thereof, use in the of assay devices and obtaining transgenic plants. AGPPase is an enzyme that catalyses hydrolysis a wide range small molecules with phosphodiester bonds, which ADPG (adenosine diphosphate glucose) stands out. The obtained from plant extracts used determining nucleoside sugar levels, based either sugar-1-phosphate released, or monophosphate products, both formed by reaction catalysed AGPPase, as well detection sulphonucleotides such 3′-phosphoadenosine 5′phosphosulphate (PAPS) adenosine (APS). Similarly, partial sequences are described amino acids enzyme, its cDNA protein deduced. Also steps purification characterisation new isoform ADPglucose pyrophosphatase associated starch granules Finally, plants over-express possess high content sugars resistance to salt concentrations.

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