UDPglucuronic acid pyrophosphatase assay with the aid of alkaline phosphatase.

摘要: A simple and sensitive method has been described for the determination of UDPglucuronic acid pyrophosphatase activity. Pyrophosphatase-free alkaline phosphatase preparation is added to reaction mixture in order hydrolyze phosphate esters (UMP alpha-D-glucuronic 1-phosphate) produced by pyrophosphatase. The inorganic liberated measured a modification Fiske SubbaRow's method. coupled time saving, easy perform accurate. It can also be used assays with other nucleotide substrates like UDPglucose, UDP-N-acetylglucosamine, NAD+, NADH, NADP+ NADPH.