New method for the quantitative assay of the enzymatic hydrolysis of udpglucuronic acid using analytical capillary isotachophoresis

摘要: Abstract The microsomal fraction of mamamalian liver homogenate contains a pyrophosphatase activity whicy catalyses the hydrolysis UDPglucuronic acid to UMP and glucuronic 1-phosphate. A further phosphatase cleaves these products uridine phosphate, respectively. new method for assay reactions is described using analytical capillary isotachophoresis. This technique permits simultaneous quantitative analysis mixtures containing acid, primary hydrolysis, 1-phosphate, secondary products, phosphate acid. sensitivity sufficient accurate determination 0.1 nmole substances applied isotachophoresis instrument in volume 1–10 μl, i , e solutions concentration as low 10 μmole/l. sample-to-sample time, including washing resetting order 40 min. major advantages this are that complete possible without need radioactively labelled compounds time considerably less than conventional chromatographic separation.