Non-lytic, non-ionic detergent extractions of plasma membrane constituents from normal and transformed fibroblasts
作者： Edward Pearlstein , John Seaver
DOI: 10.1016/0005-2736(76)90123-1
关键词:
摘要: A technique has been developed for the selective extraction of plasma membrane protein constituents from normal and transformed cells employing non-ionic detergents. The procedure does not damage as judged by cell viability, 51Cr release, trypan blue staining. Lactoperoxidase-catalyzed iodina- tion followed detergent permits demonstration a 100 000 dalton which is found on surface but hamster mouse fibroblasts.
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