Polypeptides of a glycoprotein antigen (SF) present in serum and surface of normal but not of transformed chicken fibroblasts.

摘要: Abstract A protein immunologically identical to a glycoprotein antigen from fibroblast plasma membrane (SF complex) is present in chicken serum. This disappears cells transformed with tumor viruses. The solubilized fibroblasts using urea and detergents, the serum component both gave molecular weight of about 2 · 105 as estimated by gel filtration on Sepharose 4B. Ultracentrifugation 5–20% sucrose gradients value 7–8 S for sources. Isolation extracts was achieved immunochemical techniques. Analysis polypeptide chains cellular electrophoresis presence sodium dodecylsulphate, disclosed three major components at weights 210 000 145 45 000. two high bands were identified electrophoretograms whole cell absorption subsequent elution immunoadsorbents. 000-molecular comigrated purified actin electrophoresis. After short pulses [35S]methionine polypeptides, particular, among most prominent radioactive indicating that SF has rapid turn-over. isolated showed composition similar one except not detectable.