Modulation of T cell activation by differential regulation of the phosphorylation of two cytosolic proteins. Implication of both Ca2+ and cyclic AMP-dependent protein kinases.
作者： D Mary , J F Peyron , P Auberger , C Aussel , M Fehlmann
DOI: 10.1016/S0021-9258(18)71706-7
关键词:
摘要: Interleukin 2 production by activated Jurkat T cells is markedly decreased prostaglandin E2 (PGE2). The target of PGE2 action has been investigated in the present study. Among biochemical events occurring after CD3.TCR triggering anti-CD3 monoclonal antibody, phosphorylation two cytosolic proteins, pp21 and pp23, was strongly inhibited PGE2, forskolin, 8-bromo-cAMP, whereas antibody-induced modulation Ca2+ influx were not affected. inhibition both pp23 interleukin synthesis can be largely reversed cAMP-dependent protein kinase inhibitor, N-[2-(methylamino)-ethyl-1]-5-isoquinoline sulfonamide. Together with demonstration a activity cells, these results are consistent participation mediating inhibitory probably through phosphorylation. Thus, it appears that proteins represents an essential step regulation lymphocyte activation.
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