Testing Somaclonal Variation Using RAPDs in Quercus suber L. Somatic Embryos

摘要: Somaclonal variation in Quercus suber L. somatic embryos during recurrent embryogenesis was tested by developing Random Amplified Polymorphic DNA (RAPD) profiles. Forty arbitrary 10-mer primers were used to amplify from isolated of 12 genotypes, some them belonging two half-sib families. One the genotypes also analyzed after several generations embryogenesis. RAPD patterns identical within and with time culture. The technique allowed us differentiate all genotypes. grouping based on their genetic distances using different similarity indexes, consistent known provenance embryogenic lines. absence detection somaclonal is discussed terms origin embryos.