Comparison of the value of pulsed-field gel electrophoresis, random amplified polymorphic DNA and amplified rDNA restriction analysis for subtyping Taylorella equigenitalis
作者： S. Kagawa , J.E. Moore , O. Murayama , M. Matsuda
关键词:
摘要: Eight strains of Taylorella equigenitalis were identified by a polymerase chain reaction using primer pair specific to the 16S rDNA T. equigenitalis. These eight chosen because they had previously been shown represent distinct genotypes pulsed-field gel electrophoresis analysis after separate digestion genomic DNA with ApaI or NotI. The could be classified into six seven types random amplified polymorphic different kinds primers. Amplified restriction five enzymes, including AluI and MboI, 1500 bp fragments did not discriminate variations among Thus, was these organisms better than analysis, while found unsuitable for subtyping
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