Detection of Neisseria gonorrhoeae infection by ligase chain reaction testing of urine among adolescent women with and without Chlamydia trachomatis infection.

摘要: Background and Objectives: Culture, the conventional method for detection of Neisseria gonorrhoeae, requires invasive sampling stringent specimen transport conditions. The recently developed ligase chain reaction test (LCR; Abbott Laboratories; North Chicago, IL) allows noninvasive stable conditions, but has not been evaluated with specimens from adolescent populations. Goal this Study: To perform a comparative evaluation commercial LCR culture diagnosis N. gonorrhoeae in women. Study design: Urine endocervical swab 330 teenage women seen two public health clinics were tested by culture. For resolution discordant results, polymerase (PCR) was that directly amplifies DNA urine samples processed LCR. Results: Thirty-one (9.4%) cervical culture-positive 30 (9.1%) positive After 13 sensitivity, specificity, negative predictive values 88.2%, 100%, 98.7%, respectively, 82.3%, 98.9%, 90.3% 98%, respectively. Conclusions: Although more expensive than culture, offers sensitive means may be useful purpose settings where pelvic examinations are difficult to simultaneous Chlamydia trachomatis is advantageous.