Cytokeratin 19 promoter directs the expression of Cre recombinase in various epithelia of transgenic mice
作者： Gui-Feng Zhao , Shuang Zhao , Jia-Jie Liu , Ji-Cheng Wu , Hao-Yu He
DOI: 10.18632/ONCOTARGET.15435
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摘要: // Gui-Feng Zhao 1 , Shuang Jia-Jie Liu Ji-Cheng Wu Hao-Yu He Xiao-Qing Ding Xue-Wen Yu 2 Ke-Qiang Huang Zhi-Jie Li Hua-Chuan Zheng Department of Experimental Oncology and Animal Center, Shengjing Hospital China Medical University, Shenyang 110004, Office Administration, Jinzhou 121001, Correspondence to: Zheng, email: zheng_huachuan@hotmail.com Keywords: Cre recombinase, transgenic mouse, cytokeratin 19, PTEN, carcinogenesis Received: December 01, 2016 Accepted: January 11, 2017 Published: February 17, 2017 ABSTRACT Cytokeratin 19 (K19) is expressed in various differentiated cells, including gastric, intestinal bronchial epithelial liver duct cells. Here, we generated a mouse line, K19-Cre, which the expression recombinase was controlled by promoter K19. To test tissue distribution excision activity K19-Cre mice were bred with Rosa26 reporter strain that carries PTEN conditional alleles (PTEN Loxp/Loxp ). At mRNA level, strongly stomach, lung intestine, while lung, at protein level. The immunoreactivity to observed cytoplasm detectable according LacZ staining. In K19-Cre/PTEN mice, abrogated breast, former two verified situ PCR. There appeared breast cancer loss. These data suggest K19 may be useful tool study pathophysiological functions 19-positive especially gastrointestinal Cell specificity neoplasia not completely attributable cell-specific oncogenes loss tumor suppressor genes.
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